Study on genetic diversity of 39 Citron germplasm resources with SCoT and ISSR markers

The genetic relationships and diversity of 39 citron (Citrus medica) germplasm resources were examined bySCoT and ISSR markers in this study. Of the 80 SCoT primers screened, 9 primers gave reproducible, polymorphicDNA amplification patterns with a total 69 amplified bands. The polymorphic percentage was 76.8%. Of the100 ISSR primers screened, 9 primers gave reproducible, polymorphic DNA amplification patterns with a total84 of amplified bands. The percentage of polymorphic band was 77.4%. An UPGMA relationship tree wasestablished based on the similarity coefficients. At the coefficient level of 0. 85 for SCoT and 0.77 for ISSR, the39 germplasm resources were divided into six groups and five groups, respectively. The application of SCoTand ISSR markers is discussed in this paper.