Determination of self-incompatibility status of some clementine (Citrus clementina) genotypes by his

Most of clementine mandarins belonging to Citrus that is extremely important fruit group in the world showself-incompatible features as sexual. Several cultivars, characterized for high compatibility level with othervarieties, often produce several seeds in fruits. Although seeds in mandarins-like represent a huge problemfor citrus industry, the mechanisms related to citrus incompatibility have not been deeply investigated. In thisstudy, self-compatible/incompatible status of 40 clementine mandarins located at the ‘University of Cukurova,Faculty of Agriculture, Tuzcu Citrus Collection’ were determined by histological analysis. For this purpose, self-pollination was performed and following the self-pollination, pistil samples until the fifteenth day from thethird day were taken at intervals of two days (3, 5, 7, 9, 11, 13, 15, 20th days after pollination). All samples fixedin FPA70 solution and stored at 4 °C. Pistil samples were stained with aniline blue for microscope observation. The preparations were observed by fluorescence microscope after staining and squashing. All pollen tubes ofthe genotypes arrested in the upper or middle part of style and none reached the base of the style. As a resultof investigations, 40 varieties of clementines have been identified certainly as a self-incompatible.