用rolB基因转化大红甜橙的初步研究

Internodal stem segments from 30 d old seedlings of‘Dahong’sweet orange(Citrus sinensis Osbeck)were cultured on bud induction media，to study on the genetic transformation in Citrus with rolB gene．The best medium for induction was MS+3.0 mg/L 6-BA，which led to 95%of explants producing at least one bud，so this medium could be considered as the best for bud induction in plant regeneration．The co-culture mediumⅡ(MS+3 mg/L 6-BA+200μmol/L As)+selective mediumⅣ(MT+3 mg/L 6-BA+50 mg/L Kan+500 mg/L Cef)were the suitable combination for production of transgenic bud，and on which the ratio of buds induction could be 51%．Kan-resistant buds were micro grafted on in vitro grown seedlings of C.Sinensis×P.trifoliata，and 19 transgenic plants derived there well in the greenhouse.