转phyB基因枳橙生物学特性分析

Citranges transferred with phyB gene were detected at the molecular level and their biological characteristics were analyzed.PCR,RT-PCT and PCR-Southern blot analysis indicated that phyB gene was successfully integrated into citrange genome and was transcribed in citrange.Compared with the non-transgenic plant,the transgenic plant was dwarf,with larger shoot insertion angle and smaller leaf area,and was higher in chlorophyll content,net photosynthesis rate,light saturation point and specific leaf weight.Determination of endogenous hormone by HPLC indicated that the content of GA1,GA4,ABA and Z of the transgenic plant was 32.7%,50%,121%and 17.8%higher than that of the non-transgenic plant,respectively,whereas the content of IAA was lower than that of the non-transgenic plant.(GA1+GA4)/ABA and Z/ABA ratios of the transgenic plant were 38.4%and 43.2%lower than those of the control,respectively.