绿色荧光蛋白基因转化柑橘胚愈伤组织与高效再生

Transformation ofgreen fluorescent protein gene(gfp)intocitrus embryogenic calluses of13 cultivars mediated by Agrobacteriumtumefaciens was conducted.Totally82 transgenic lines from11 cultivars were produced,among which 28 and 25 transgenic lines were obtained from Frost navel orange and Sunburst tangerine respectively.Further PCR analysis of 3 selected transformed cultivars amplified the expected GFP fragment.This study proved that GFP as a vital marker could localize the transgene sites at an early stage,be helpful for discriminating escapes and chimeras,and physically separating in vitroputative transformants.High level ofgfp expression did not affect transgenic cell division,somatic embryogenesis and further differentiation.These transgenic cell lines are valuable materials in citrus basic research and currently being used in studies on citrus somatic fusion and social control mechanismbetween calluses ofdifferent ploidylevels.