农杆菌介导phyB基因转化枳壳研究

The phyB gene was transferred into the genome of Poncirus trifoliate in vitro with explants of epicotyl mediated by Agrobacterium．To诅l 27 strains with plant resistance plants were regenerated．The presence of specific targeted strips of phyB gene in the 5 regenerated plants Was confirmed by PCR analysis．RT-PCR analysis indicated the regular expression of transgene phyB in two transgenic clones，phyB3 and phyB4 in omparison with the control plants，the phyB transgenic plants had higher net photosynthesis rate．All the regenerated transgenic plants，as compared with the untransformed clones，showed lower height，shorter interval between intemodes and smaller leafarea．