原生质体电融合再生柑橘属间四倍体体细胞杂种

Mesophyll protoplasts of Changshou kumquat (Fortunella obovata) were fused electrically with embryogenic protoplasts of Dancy tangerine (Citrus reticulata). Plants were obtained after culture of the fusion treated protoplasts. Cytological examinations showed that the plants were tetraploids with 36 chromosomes. RAPD analysis indicated that the plants had bands specific to the fusion partners in the primers (OPA 04 and OPA 07) chosen for verifying hybridity of the plants. The results demonstrated that tetraploid intergeneric somatic hybrid plants have been recovered by fusion of protoplasts between kumquat and citrus.