粗柠檬与哈姆林甜橙二倍体体细胞杂种胞质基因组初步分析

RFLP (restriction fragment length polymorphism) was employed to analyze cytoplasmic genome of diploid somatic hybrid plant, morphologically similar to rough lemon which was leaf parent, that was produced via protoplast fusion between rough lemon ( Citrus jambhiri Lush) and Hamlin sweet orange ( C. sinensis Osb.), the embryogenic parent. Three enzyme_mitocondrial probe combinations and one enzyme_chloroplast probe combination demonstrated that the plant had identical band profiles to Hamlin sweet orange as far as mtDNA and cpDNA were concerned.