Construction of the plant expression vector with hepatitis A capsid protein fusion gene and genetic transformation of Citrus sinensis Osbeck

The use of edible for the production and delivery of vaccine could provide an economical alternative to systems. The construction of the plant expression vector pBI121-A was reported, which contained a fusion gene encoding A . The gene was located between the left and right Ti border sequences under the control of CaMV35S promoter. The vector was identified via PCR and restriction enzyme analysis and was introduced into tumerifacience LBA4404. The transgenic were produced by -mediated transformation of epicotyl segments.13 putatively transformed through the selection were micrografted onto the seedlings. The presence and integration of the transgene had been verified by PCR analysis. The result showed that five transformants were integrated and the transformation efficiency was 4.1%.