Molecular Verification of Putative Zygotic Seedlings in Different Intra-Specific Crosses in Mandarins (Citrus reticulata) by SSR Markers

The molecular verification of different intra-specific mandarin hybrids was explored during 2014-2015. Simple sequence repeat (SSR) markers were employed to identify putative zygotic seedlings from hybrid progeny produced from different crosses in mandarin. The most informative SSR marker in the F-1 hybrids of "Daisy x Kinnow' cross was CCSM170 which identified 73% of zygotic seedlings followed by CMS4, AG14, CiBE6006 and CiBE3397 which identified 38.5%, 31.5%, 27% and 24% of zygotic seedlings, respectively. Most efficient SSR marker in "Kinnow x Daisy' cross was CiBE6006 which identified 22.5% zygotic seedlings followed by CCSM170, CiBE3397 and CMS4 which identified 17.5%, 12.5% and 5.5% of zygotic seedlings, respectively. In 'W. Murcott x Kinnow' hybridization, CMS31 marker identified 28.5% zygotic seedlings in F-1 progeny followed by CiBE3298 which identified 14.5% zygotic seedlings. The SSR marker CiBE3397 identified 19.5% zygotic seedlings in 'Kinnow x W. Murcott' cross. The results show that the SSR markers can differentiate the zygotic seedlings of F-1 progeny in different intra-specific crosses in mandarins. <br />