Amplification of the Full-Length PAMP Gene and Difference of the mRNA Expression Among Three Lean Pi

To understand the function of porcine adipocyte-special membrane protein (PAMP) gene and the difference of fat deposition ability among various lean pig breeds, a full-length porcine adipocyte-special membrane protein (PAMP) gene was successfully amplified using reverse transcription polymerase chain reaction (RT-PCR) and 5′-rapid amplification of cDNA end (5′-RACE). The open reading frame was 1 587 bp encoding 529 amino acids. The nucleotide sequence of the full- length PAMP gene was deposited in the GenBank under the accession number EF433431. The PAMP gene mRNA expression was analyzed on three lean pig breeds by quantitative reverse transcription polymerase chain reaction (QRT- PCR). The PAMP gene mRNA levels in YHM (Yorkshire × Hampshire × Meishan) pig and DLY (Duroc × Landrance × Yorkshire) pig were about 0.82 and 0.38 times of that in SW (Shanxi-White) pig, respectively.