PCR Amplication and Sequencing of ITS-2 rDNA of Leucocytozoon caulleryi

<正> The second internal transcribed spacer (ITS-2) of Leucocytozoon caulleryi was amplified by PCR using a pair of conserved primers and cloned into the T-T windows of plasmid pGEM-T easy vector. The inserts were successfully sequenced and the results revealed that the ITS-2 plus flanking sequence (ITS2+) was composed of 270 nucleotides. and the ITS-2 was 113 bp in length. The ITS-2 of L. caulleryi was analysed by NCBI Blast, and the degree of homology of the ITS-2 of L. caulleryi was compared with that of Eimeria tenella , Candida tropicalis and Saccharomyces kluyveri and others by wDNASIS. The results showed that the ITS-2 of L. caulleryi is characteristic and has the greatest similarity with E. tenella (21.2%).