AFLP and RAPD Analysis of the Boer and Indigenous Breeds of the Goat in Jiangsu

<正> Blood and tissue samples were collected from 105 goats including 60 Boer goats (30 for each sex), 30 Xuhuai goats (15 for each sex) and 15 Haimen goats (7 stud and 8 does). DNA was extracted and DNA pools were constructed on the basis of goat breeds. In 36 selective primer combinations, 29 combinations amplified totally 3 253 markers including 92 polymorphic markers by amplified fragment length polymorphism (AFLP). On average, 3.17 polymorphic markers were amplified per combination, with a polymorphic frequency of 2. 8%. The primer combinations amplifying more polymorphic markers (showed in brackets) were involved in E00+ACG/M00+CAA (13), E00+ACG/M00+CAG (10), E00+AAC/M00+CAC (8) and E00+A AC/M00+ACT (7). A total of 183 markers including 60 polymorphic markers were amplified by RAPD from the pooled DNA of three breeds using 22 primers with strong polymorphism and high reproduc-ibility selected from 93 RAPD primers. On average, 2. 73 polymorphic markers were amplified per primer, with a polymorphic frequ