Expression and Localization of HSPs in the Liver of Transported Pigs

<正> To demonstrate the distribution and expression of heat shock proteins (HSPs) in the liver, five kinds of HSPs, namely HSP70, HSP72, HSP86, HSP90 and HSP27 were determined after a long distance transport using immunohistochemistry and Western blot. All five HSPs were regularly detected in the liver of both the transported and the control group. Both nuclear and cytoplasmic labeling was present in hepatic cells; however, localization of HSPs was quite different in hepatocytes. The majority of HSP70, HSP72 and HSP27 appeared to predominate in cytoplasm of the hepatocytes. Nuclear signals of HSP90 and HSP86 were most prominent in hepatocytes in which immunostaining in the cytoplasm was relatively weak compared with that in the nucleus. There was subjectively lower staining in the edge of hepatic labule, and lower numbers of HSPs labeled hepatic cells corresponded to light microscopic lesions. The obvious inductions of some HSPs were detected in the liver, especially the HSP90(P<0.01) which belongs to the HS