农杆菌介导的GUS基因在枳壳中的转移

The transformation of GUS gene byAgrobacterium-mediation was studied. The explants used for transformation were the epicotyls fromPoncirus trifoliataRaf. The results indicated that Cefotaxime used as antibiotics was better than carbenicillin. The concentration of kanamycin used as the reagent of choice was 50 mg/L. Shoot regeneration frequency was high when cocultivation time was 2~3 days. Histochemical GUS assay showed that 80.0% of the explants had the transient expression of GUS gene and 70.0% of the resistant plants were GUS-positive.