农杆菌介导红江橙遗传转化的研究初报

The transformation of GUS gene byAgrobacterium-mediated was studied. The explants used for transformation were the epicotyls fromCitrus sinensisOsbeck cv.Hongjiang. The results indicated that the concentration of kanamycin used as the reagent of selection was 50 mg/L; when the explants were cultured on the selection medium, they placed horizontally better than they oriented with their apical ends protruding from the medium. Cefotaxime used as antibiotics was better than carbenicillin. Histochemical GUS assay showed that 70.4% of the explants had the transient expression of GUS gene and 16.7% of the 12 resistant plants were GUS-positive.