根癌农杆菌介导甲型肝炎病毒(HAV)衣壳蛋白融合基因转化柑桔成年材料的研究

With the mature axillary buds of citrus excellent variety Shinanui(Citrus Reticulate×Citrus sinensis) severed as the explant,The plantlets which carried the hepatitis a virus capsid protein fusion gene were obtained through Agrobacterium-mediated transformation.In this study,survival in graft of mature axillary buds of shinanui was the highest,up to 90%,when the orange rootstocks were cultured 15 days.Sterilization time was effected on maturity of shinanui branches.The more maturity of branches,the longer of sterilization time was.The putative transgenic plantlets was selected by PCR,PCR amplification product of PCR positive plantlets were sequenced, and the results showed that Hepatitis a virus capsid protein fusion gene was integrated into genome of transgenic shinanui.