金柑遗传体系的建立及应用

An effieient in vitro regeneration system and Agrobacterium-mediated transformation system were established in kumquats (Fortunella crassifolia swingle), and more than 20 transgenic plants were obtained. Three protoeols, multiplex PCR,T一DNA flanking sequence analysis and Southern blotting, were applied to early, middle and late analysis of transformation，respectively. With the established transformation system, antisense orange β一earotene hydroxylase gene was introduced into kumquat in order to produce high β一earotene fruits. Meanwhile, transgenic plants incorporating GUS gene under an orange chlorophyllase gene promoter were obtained to evaluate the expression pattern of the promoter.