用单性结实基因defH9-iaaM转化糖橙的研究

A parthenocarpy gene(DefH9-iaaM)was introduced into Succari to obtain possible seedless transformants, by using internodal stem segments as explants to establish an efficient regeneration and transgenic system.The results indicated that 30-day-old seedlings were the most efficient explants.The co-culture in darkness for 3~6 days gave a rise to good regeneration.The optimum medium for adventitious buds regeneration was MS+6-BA 5.0 mg/L.And MS medium supplied with 6-BA1.0 mg/L and NAA0.1 mg/L showed the highest bud multiplication rate，3.3 buds on each internodal segments.The shoots successfully rooted after 20 days in 0.5 mg/L NAA.After Agrobacterium-mediated transformation，6 transgenic plants out of 17 regenerants were identified by PCR.