<正> The effects of the concentration of MS micro salts, 2,4-dichlorophenoxyacetic acid (2,4-D), 3-naphthalene acetic acid (NAA), proline and adenine on callus formation and somatic embryogenesis were investigated using orthogonal design with immature cotyledons of soybean. The results showed that the role of concentration of micro salts on frequency of callus formation and somatic embryogenesis were significant. MS

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