柑桔裂皮类病毒江西分离物（CEVd-RJ）全长cDNA序列分析

运用RT-PCR技术对柑桔裂皮类病毒江西分离物CEVd-RJ的全长cDNA序列进行扩增、克隆,并对其片段进行序列测定。将获得的371bp的全长cDNA序列与GenBank登录的11个柑桔裂皮类病毒相应序列进行比对分析,结果发现CEVd-RJ与湖北分离物CEVd-HB的同源性为99.7%,与广东分离物CEVd-YC的同源性为90.8%,同国外9个分离物间的同源性在89.0%～98.6%之间。序列差异分析发现,CEVd-RJ的全长cDNA序列比CEVd-YC少一个碱基,有36个位点碱基发生了变化,与CEVd-HB只存在一个碱基位点的差异。 The Citrus exocortis viroid,CEVd-RJ,isolated from Jiangxi province was amplified using RT-PCR,and the full length cDNA was cloned and sequenced.Comparison with the 11 citrus exocortis viroid isolates deposited in GenBank revealed that the Jiangxi isolate,CEVd-RJ,shared 99.7% of identity with the Hubei isolate,CEVd-HB,and 90.8% of identity with the Guangdong isolate,CEVd-YC,in their cDNA sequences.CEVd-RJ also shared 89.0% to 98.6% cDNA identities with 9 isolates from other countries.Compared with CEVd-YC,CEVd-RJ had a 1 bp deletion and 36 nucleotide changes in its full length cDNA.Notably,there was only one nucleotide difference between CEVd-RJ and CEVd-HB.