转rolA、B、C基因枳橙快繁技术

In vitro propagation makes it possible to speed the multiplication of the rol A，B，C transgenic citrange plants，SO it is possible to quickly evaluate this new rootstock．Using bud sticks of the transgenic clones(B，D，E)and the control citrange as explants，culture media containing various plant growth regulators were tested for bud bumt，shoot multiplication and rooting．The results indicated that MS medium containing 1 mg／L 6-BA resulted in fastest bud bumting，MS added with 6-BA 0．5 mg／L+ NAA 0．05 mg／L was the best choice for shoot multiplication．and 1／2MS supplied with NAA 0．5 mg／L and 0．2％ activated charcoal showed quite suitable for rooting． Fhe transgenic clones had significantly higher rooting percentage (93．3％)than that of the control(66．7％)．