转rol基因枳橙分子鉴定及部分生物学的观测

Molecular identification, physiology evaluation and morphological observation of therolA, rolB androlC transgenic citrange clones (B, D, E) were conducted. PCR analysis indicated that therolA, rolB,rolC geneswere integrated into the citrange genome. The expression of the transgenesnptⅡandrolC was detected by usingRT-PCR; while the transgenesrolA androlB did notshow anyRT-PCR produc,t and it wasnotclear for the factors related to theirexpression. In comparisonwith the controlplants, theroltransgenic plantshowed evidentdwarfing characteristics, such as only halfof the control in plantheigh,t shorter internodal length, weakerapicaldominancewithmore lateralbranches, smaller leafarea, and highernetphotosyn-thesis rate. Higher contents of the endogenousGA3, IAA and zeatinwere found in themiddle and basalparts of the transgenic shoots than the contro,l this could be one of the factors responsible for the above described morphologicalmodifications.