农杆菌介导的抗菌肽A基因转入沙田柚影响因素研究

Transformation and regeneration ofCitrusgrandisplants were studied byAgrobacterium-mediated. Transgenic plants with ShivaA gene had been obtained. The carbenicillin was better to controlAgrobacterium growing after explants were infected. Epicotyls fragment were infecting for 10 min as OD600ofAgrobacterium suspending liquid is 0.5. Epicotyls fragment were co-cultured withAgrobacteriumfor 3days. The transformation frequency of explants was promoted when 150μmol/L ferulic acid was added inAgrobacteriumsuspension liquid & co-culture medium. It was useful for anti-Kan positive shoot producing when initial concentration of kanmaycin was 50μg/mL. Foreign gene is proved going toC.grandisby PCR & Southern blot detective